ARTIGOS

2020

HIDRÓLISE ENZIMÁTICA

Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

Ayla Sant’Ana da Silva, Roberta Pereira Espinheira,  Ricardo Sposina Sobral Teixeira, Marcella Fernandes de Souza, Viridiana Ferreira-Leitão e Elba Pinto da Silva Bon

Biotechnology for Biofuels. , v.13, p.58 - , 2020.

2019

CARACTERIZAÇÃO

Physicochemical characterization of residual biomass (seed and fiber) from açaí (Euterpe oleracea) processing and assessment of the potential for energy production and bioproducts.

Anna Cristina Pinheiro de Lima, Dandara Leal Ribeiro Bastos, Mariella Alzamora Camarena, Elba Pinto da Silva Bon, Magali Christe Cammarota, Ricardo Sposina Sobral Teixeira e Melissa Limoeiro Estrada Gutarra.

Biomass Conversion and Biorrefinery. , v.1, p.11 - , 2019.

PRÉ-TRATAMENTO

Triticum spelta straw pretreatment for the production of glucose syrups via enzymatic hydrolysis.

Rodrigo da Rocha Olivieri de Barros, Paolo Becarelli, Raul Alves de Oliveira, Leonardo Tognotti e Elba Pinto da Silva Bon.

 

Biochemical Engineering Journal, v. 151, 107340, 2019.

PURIFICAÇÃO DE PROTEÍNAS

Expression, purification, and characterization of asparaginase II from Saccharomyces cerevisiae in Escherichia coli.

Wagner Lopes, Bárbara Adriana Ferreira dos Santos, André Luiz Franco Sampaio, Ana Paula Gregório Alves Fontão, Hilton Jorge Nascimento, Patrícia Barbosa Jurgilas, Fernando Araripe Gonçalves Torres, Elba Pinto da Silva Bon, Rodrigo Volcan Almeida e Maria Antonieta Ferrara.

 

Protein Expression and Purification, v. 159, p. 21-26, 2019.

ENZIMAS

Use of grape pomace for the production of hydrolytic enzymes by solid-state fermentation and recovery of its bioactive compounds.

Aline S. C. Teles, Davy W. H. Chávez, Raul A. Oliveira, Elba P. S. Bon, Selma C. Terzi, Erika F. Souza, Leda M. F. Gottschalk e Renata V. Tonon.

 

Food Research International, v. 120, p. 441-448, 2019.

PRÉ-TRATAMENTO

Modification of microcrystalline cellulose structural properties by ball-milling and ionic liquid treatments and their correlation to enzymatic hydrolysis rate and yield.

Felipe Tadeu Fiorini Gomide, Ayla Sant’Ana da Silva, Elba Pinto da Silva Bon e  Tito Lívio Moitinho Alves.

 

Cellulose, v. 26, p. 7323–7335, 2019.

MICROALGAS

Interference of starch accumulation in microalgal cell growth measurement.

Marcella Fernandes de Souza, Marcoaurélio Almenara Rodrigues, Elba Pinto da Silva Bon e Suely Pereira Freitas.

 

Journal of Applied Phycology, v. 31, p. 249-254.

2018

ENZIMAS

A novel Trichoderma harzianum strain from Amazon Forest with high cellulolytic capacity

Marcella de Souza Fernandes, Ayla Sant’ana da Silva e Elba Pinto da Silva Bon

Biocatalysis and Agricultural Biotechnology 2018, V. 14, p.183-188

PROCEDIMENTOS ANALÍTICOS

Colorimetric protein determination in microalgae (Chlorophyta): association of milling and SDS treatment for total protein extraction.

Maria Fernanda S. Mota, Marcella de Souza Fernandes, Elba Pinto da Silva Bon, Marcoaurélio Almenara Rodrigues e Suely Pereira Freitas

 

Journal of Phycology, 2018, V. 54, p. 577-580

2017

HIDRÓLISE ENZIMÁTICA

One-vessel saccharification and fermentation of pretreated sugarcane bagasse using a helical impeller bioreactor

Raul Alves de Oliveira, Leda Maria Fortes Gottschalk, Suely Pereira Freitas e Elba Pinto da Silva Bon

 

Biomass conversion and Biorefinery, 2017, V. 8, Issue 1, pp 1-10

PRÉ-TRATAMENTO

Continuous pretreatment of sugarcane biomass using a twin-screw extruder

Mariana Kuster Moro, Ricardo Sposina Sobral Teixeira, Ayla Sant’ana da Silva, Maristela Duarte Fujimoto, Príamo Albuquerque Melo e Argimiro Resende Secchi

 

Industrial Crops and Products, 2017, V. 97, P. 509-517

PROCEDIMENTOS ANALÍTICOS

Neutral sugars determination in Chlorella: Use of a one-step dilute sulfuric acid hydrolysis with reduced sample size followed by HPAEC analysis

Marcella Fernandes Souza, Daniel Santos Pereira, Suely Pereira Freitas, Elba Pinto da Silva Bon e Marcoaurélio Almenara Rodrigues

 

Algal Research: Biomass, Biofuels and Bioproducts, 2017, V. 24, Part A, P. 130-137

BIOTECNOLOGIA

Synthesis of disaccharides using β-glucosidases from Aspergillus niger, A. awamori and Prunus dulcis

Ayla Sant’ana da Silva, Javier Freddy Molina, Ricardo Sposina Sobral Teixeira, Luis G Valdivieso Gelves, Elba Pinto da Silva Bon e Viridiana Santana Ferreira-Leitão

 

 

Biotechnology Letters, 2017, V. 39, P. 1717-1723

2016

HIDRÓLISE ENZIMÁTICA

High-solids content enzymatic hydrolysis of hydrothermally pretreated sugarcane bagasse using a laboratory-made enzyme blend and commercial preparations

Ayla Sant’ ana da Silva, Marcella Fernandes de Souza, Ignacio Ballesteros, Paloma Manzanares, Mercedes Ballesteros e Elba Pinto da Silva Bon

 

Process Biochemistry, 2016, V. 51, Issue 10, P. 1591-1567

2015

MICROALGAS

Untreated Chlorella homosphaera biomass allows for high rates of cell wall glucan enzymatic hydrolysis when using exoglucanase-free cellulases

Marcoaurélio Almenara Rodrigues, Ricardo Sposina Sobral Teixeira, Viridiana Santana Ferreira-Leitão, Elba Pinto da Silva Bon

 

Biotechnology for Biofuels 2015, 8:25

ENZIMAS

Production of xylanase, α-l -arabinofuranosidase, β-xylosidase, and β-glucosidase by Aspergillus awamori using the liquid stream from hot-compressed water treatment of sugarcane bagasse

Raquel de Sousa Paredes, Rodrigo da Rocha Olivieri de Barros, Hiroyuki Inoue
Shinichi Yano, Elba Pinto da Silva Bon

 

Biomass Conversion and Biorefinery 2015

NANOCRISTAIS

Combining biomass wet disk milling and endoglucanase/β-glucosidase hydrolysis for the production of cellulose nanocrystals

Ricardo Sposina Sobral Teixeira, Ayla Sant’Ana da Silva, Jae-Hyuk Jang, Han-Woo Kim, Kazuhiko Ishikawa, Takashi Endo, Seung-Hwan Lee, Elba Pinto da Silva Bon

 

Carbohydrate Polymers 2015, 128:75–81

2014

HIDRÓLISE

Enzymatic hydrolysis of sugarcane bagasse and straw mixtures pretreated with diluted acid

Rondinele De Oliveira Moutta, Viridiana Santana Ferreira-Leitão, Elba Pinto Da Silva Bon

Biocatalysis and Biotransformation 2014, 32(1)

BIOCONVERSÃO

Bioconversion of R-(+)-limonene to perillic acid by the yeast Yarrowia lipolytica

Maria Antonieta Ferrara, Débora S. Almeida, Antonio C. Siani, Leonardo Lucchetti, Paulo S.B. Lacerda, André Freitas, Marcelo R.R. Tappin, Elba Pinto da Silva Bon

Brazillian Journal of Microbiology 2014, 4

2013

ENZIMAS

Efficient production of lignocellulolytic enzymes xylanase, β-xylosidase, ferulic acid esterase and β-glucosidase by the mutant strain Aspergillus awamori 2B.361 U2/1

Leda Maria Fortes Gottschalk, Raquel de Sousa Paredes, Ricardo Sposina Sobral Teixeira, Ayla Sant’Ana da Silva, Elba Pinto da Silva Bon

Brazillian Journal of Microbiology 2013, 44(2)

PRÉ-TRATAMENTO

Association of wet disk milling and ozonolysis as pretreatment for enzymatic saccharification of sugarcane bagasse and straw

Rodrigo da Rocha Olivieri de Barros, Raquel de Sousa Paredes, Takashi Endo, Elba Pinto da Silva Bon, Seung-Hwan Lee

Bioresource Technology 2013, 136:288–294

PRÉ-TRATAMENTO

Continuous pretreatment of sugarcane bagasse at high loading in an ionic liquid using a twin-screw extruder

Ayla Sant’Ana da Silva, Ricardo Sposina Sobral Teixeira, Takashi Endo, Elba Pinto da Silva Bon, Seung-Hwan Lee

Green Chemistry 2013, 15:1991-2001

PRÉ-TRATAMENTO

Comparative Response and Structural Characterization of Sugarcane Bagasse, Straw and Bagasse-Straw 1:1 Mixtures Subjected To Hydrothermal Pretreatment and Enzymatic Conversion

Rondinele de Oliveira Moutta, Maria Cristina Silva, Roberta Cristina Novaes Reis Corrales, Maria Alice Santos Cerullo, Viridiana Santana Ferreira-Leitão, Elba Pinto da Silva Bon

Journal of Microbial and Biochemical Technology 2013, S12

HIDRÓLISE

Use of cellobiohydrolase-free cellulase blends for the hydrolysis of microcrystalline cellulose and sugarcane bagasse pretreated by either ball milling or ionic liquid |Emim||Ac|

Ricardo Sposina Sobral Teixeira, Ayla Sant’Ana da Silva, Han-Woo Kim, Kazuhiko Ishikawa, Takashi Endo, Seung-Hwan Lee, Elba Pinto da Silva Bon

Bioresource Technology 2013, 149:551–555

2012

PROCEDIMENTOS ANALÍTICOS

Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements

Ricardo Sposina Sobral Teixeira, Ayla Sant’Ana da Silva, Viridiana Santana Ferreira-Leitão, Elba Pinto da Silva Bon

Carbohydrate Research 2012, 363:33–37

2011

MICROALGAS

Evaluation of Chlorella (Chlorophyta) as source of fermentable sugars via cell wall enzymatic hydrolysis

Marcoaurélio Almenara Rodrigues, Elba Pinto da Silva Bon

Enzyme Research 2011

PRÉ-TRATAMENTO

Major improvement in the rate and yield of enzymatic saccharification of sugarcane bagasse via pretreatment with the ionic liquid 1-ethyl-3-methylimidazolium acetate |Emim||Ac|

Ayla Sant’Ana da Silva, Seung-Hwan Lee, Takashi Endo, Elba Pinto da Silva Bon

Bioresource Technology 2011, 102(22):10505–10509

HIDRÓLISE

Sugarcane bagasse enzymatic hydrolysis: rheological data as criteria for impeller selection

Leonardo Tupi Caldas Pereira, Lucas Tupi Caldas Pereira, Ricardo Sposina Sobral Teixeira, Elba Pinto da Silva Bon, Suely Pereira Freitas

Journal of Industrial Microbiology & Biotechnology 2011, 38(8):901-907

2010

ENZIMAS

Purification and characterization studies of a thermostable β-xylanase from Aspergillus awamori

Ricardo Sposina Sobral Teixeira, Félix Gonçalves Siqueira, Marcelo Valle de Souza, Edivaldo Ximenes Ferreira Filho, Elba Pinto da Silva Bon

Journal of Industrial Microbiology & Biotechnology 2010, 37(10):1041-1051

ENZIMAS

Production of cellulolytic enzymes by fungi Acrophialophora nainiana and Ceratocystis paradoxa using different carbon sources

Rodrigo Rocha de Oliveira Barros, Raul Alves de Oliveira, Leda Maria Fortes Gottschalk, Elba Pinto da Silva Bon

Applied Biochemistry and Biotechnology 2010, 161(1-8):448-454

PRÉ-TRATAMENTO

Milling pretreatment of sugarcane bagasse and straw for enzymatic hydrolysis and ethanol fermentation

Ayla Sant’Ana da Silva, Hiroyuki Inoue, Takashi Endo, Shinichi Yano, Elba Pinto da Silva Bon

Bioresource Technology 2010, 101(19):7402–7409

HIDRÓLISE

Cellulases, xylanases, β-glucosidase and ferulic acid esterase produced by Trichoderma and Aspergillus act synergistically in the hydrolysis of sugarcane bagasse

Leda Maria Fortes Gottschalk, Raul Alves Oliveira, Elba Pinto da Silva Bon

Biochemical Engineering Journal 2010, 51(1–2):72–78

REVISÕES

Biomass Residues in Brazil: Availability and Potential Uses

Viridiana Ferreira-Leitão, Leda Maria Fortes Gottschalk, Maria Antonieta Ferrara, Alexandre Lima Nepomuceno, Hugo Bruno Correa Molinari, Elba Pinto da Silva Bon

Waste and Biomass Valorization 2010, 1(1):65-76

Biotechnology for Biofuels. , v.13, p.58 - , 2020.

HIDRÓLISE ENZIMÁTICA

ENZIMAS

PROCEDIMENTOS ANALÍTICOS

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Interference of starch accumulation in microalgal cell growth measurement.

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Biotechnology for Biofuels. , v.13, p.58 - , 2020.